2021.12.05 08:41 Consistent_Meat_9211 Upcoming RPG SMP
₊˙+. M Y S A ✧ ! : grinders, going fast-paced, or you prefer to be alone, please find another realm.
‣ Let me introduce to you an up-coming Minecraft Bedrock realm! Theme is strictly medieval(skins, builds, attitude), following a roleplay theme filled with empires, lore, dungeons, and more! We own a custom map, 5k by 5k blocks. The objective? Working with your empire to defeat the dragon. Easy peasy, right?
SERIOUS PLAYERS ONLY !!
AGREEMENT TO YOU FROM MYSA :
· · We work everyday to become a better community, giving you a safe haven to call your safe space, all in our Discord server.
2021.12.05 08:41 Shizuru_Viola2010 Maybe everything does look better in black and white. :)
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2021.12.05 08:41 kvrtka Like a lot this moment Harrison joke about they india trip
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2021.12.05 08:41 art_vildtgrube Art_vildtgrube on Twitter.................................
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2021.12.05 08:41 xXCumLordXx Alt tabbing on windows 11 disconnects me from Sea of thieves
Well not me but a friend, he has w11 and he can't alt tab or he will get disconnected. This video we thought would be the solution, but he can't solve it because the "apps in background" or whatever setting that's supposed to fix this issue isn't on windows 11 yet, so we're out of ideas.
And yes he used to play fine on windows 10 with no issues. Any help is appreciated
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2021.12.05 08:41 jbzfrench [XB1] H: Fixer AAE15crit, MUE25lvc — Hm AA25ffr/15fr, MU25lvc — Lever BE25 W: Hm BE15fr, V2515fr
2021.12.05 08:41 udonnnnnn [26F] First proper selfie after haircut! Back to brown after blonde stint.
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2021.12.05 08:41 TheEvilWitch The Evil Witch it's open world, it's survival, it's rpg, it's modern pixelart, it's horror
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2021.12.05 08:41 FuckGenZ Who’s your least favourite character and why?
2021.12.05 08:41 Habundia Prosecution Gets Heated Questioning Josh Duggar Relative and Colleague: 'You Were Hiding Something'
[Prosecution Gets Heated Questioning Josh Duggar Relative and Colleague: 'You Were Hiding Something'])https://people.com/tv/josh-duggar-trial-prosecution-gets-heated-questioning-relative-colleague/)
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2021.12.05 08:41 Narfhole Bet Vin can't wait to play this gacha game!
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2021.12.05 08:41 Resident_Prior_9749 🚨 🚨 🚨 🚨 🚨 🚨 🚨 🚨 🚨 🚨 15,074 #BTC (744,709,780 USD) transferred from unknown wallet to unknown wallet https://t.co/GrDAWGcfv7
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2021.12.05 08:41 rimjob_rabbi I remixed an unreleased Uzi song with a CashMoneyAP beat, let me know what you guys think (Mixing is best after 1:45)
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2021.12.05 08:41 Saleno9 What’s some cool dinos/reptiles you want to see in a later DLC?
Personally, I’d want to see the Ostafrikosaurus, argentinasuaurs, archelon, quetzalcoatlus and uthraptor.. definitely butchered the spellings lol
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2021.12.05 08:41 nymph-x anyone else exhausted
I'm just really sick of how pro lifers go on about things every single point you make to them about why it's important to keep abortions legal because they're health care they always go back to saying the same thing "it's murder" "you're killing a child" they honestly go on about it as if it's something so brutal and traumatic when I think we all know that potentially being forced to stay pregnant because of the laws that they want will be more traumatic than never being born at all and when you tell them that you can't know you're dying if you haven't even been born yet they start telling you that it's dehumanising or start trying to define what being alive means Just generally I feel like they twist and blur the lines so hard to support their views it's just like taking to a brick wall,,
same thing over and over again
they act entitled to a potential baby that isn't even theirs I don't get where that logic comes from ?? but fml it makes me rage, the only person entitled to the potential baby is the person who's pregnant, not some stranger who blurred the lines between the end of a pregnancy and murder
idk but everytime I even read anything I see on the prolife subreddit I just start feeling drained and angry
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2021.12.05 08:41 Korina-chan I present you my abrodged thesis
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2021.12.05 08:41 moondinho Zekrom on me 6835 5792 5786
2021.12.05 08:41 boban111 Plant yourself so deeply in gratitude that even the greatest of landslides cannot shake your peace. Nicole Addison
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2021.12.05 08:41 Esepapichulo253 I really just need a hug that’s it. I’m going thru something rn. Tacoma Washington. I’m a guy just turned 21.
2021.12.05 08:41 Resident_Prior_9749 🚨 🚨 25,500,000 #USDT (25,500,000 USD) transferred from unknown wallet to Compound Tether https://t.co/DOrxrElF08
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2021.12.05 08:41 Breskvar Recommendations for protein sample desalting before native LC-MS analysis
I am working towards developing native SEC-MS and IEX-MS methods for the analysis of biopharmaceuticals, although at this point my focus is on working with a small selection of model proteins ranging from about 15 kDa to about 70 kDa in order to build a good foundation of knowledge and experience for further work.
I've managed to optimize the source and ion optics settings for all of my proteins (confirmed by the MS manufacturer's application specialists), however despite this I'm still having issues with achieving the sensitivity I'm aiming for on two of the largest proteins - ovalbumin and BSA. Looking at the acquired spectra, the application specialist and myself have come to the conclusion that the reason is the low purity and high salt content of the ovalbumin and BSA samples (sodium and potassium adducts for days, leading to loss of isotope resolution and signal distribution over many different adduct species).
Because I'd like to avoid buying reference grade materials at this point and because a sample desalting procedure will be very useful for me in the future, I'd like to get some recommendations for protein desalting products other labs are using. Obviously non-denaturing conditions are mandatory, so no RP-based cleanup.
I'm thinking something along the lines of size-exclusion protein desalting spin columns or pipette filter tips, like the Micro Bio-Spin columns by BioRad that I've seen mentioned in some app notes.
Can anyone with more experience in large molecule analysis recommend me any products that your lab is using?
I've already contacted several distributors but I'd be happy to get user advice while I'm waiting for the replies.
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2021.12.05 08:41 Gmamdmasmer Reshiram Raid first five, please be online and ready 6681 3830 9776
2021.12.05 08:41 paranoidostrich S'Wort zum Sunntig. Amen. 🤡
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2021.12.05 08:41 Zabako Trying out Tinder for the first time, all advice is welcome !
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2021.12.05 08:41 mrdyhalo "Opposites attract" - how far does this statement apply to your relationships?^